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This service is now closed. The API is no longer functional.
It's magnificently complicated!
http://twitterblacklist.com/api?username
On the blacklist? You get "yes". Not on the blacklist? You get "no".
NEW! API for humans:
http://twitterblacklist.com/api?human=1;who=username
Try it out. There's also a JavaScript bookmarklet that you can use! Drag this link onto your bookmarks toolbar for a handy spammer checking utility: Twitter Blacklist Check.